Enzymatic reactions in the production of biomethane from organic waste

Enzymatic reactions refer to organic reactions catalyzed by enzymes. This review aims to enrich the documentation relative to enzymatic reactions occurring during the anaerobic degradation of residual organic substances with emphasis on the structures of organic compounds and reaction mechanisms. This allows understanding the displacement of electrons between electron-rich and electron-poor entities to form new bonds in products. The detailed mechanisms of enzymatic reactions relative to the production of biomethane have not yet been reviewed in the scientific literature. Hence, this review is novel and timely as it discusses the chemical behavior or the reactivity of different functional groups, thereby allowing better understanding the enzymatic catalysis in the transformations of residual proteins, carbohydrates, and lipids into biomethane and fertilizers. Such understanding allows improving the overall biomethanation efficiency in industrial applications.


Topwe Mwene-Mbeja, Amélie Dufour, Céline Vaneeckhaute, Joanna Lecka, and Brar

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