Journal of Organic & Inorganic Chemistry

Abstract

Uric acid is mainly produced from metabolism of purine nucleotide in human body and have several medical importance in human system. It is therefore very imperative to develop a sensitive and accurate method of its detection. This study aimed to develop uric acid biosensor based on polydopamine-co-polypyrole (PDA-co-PPY) nanocomposite decorated on glassy carbon electrode. The conductive polymer PDA/PPY was electrochemically synthesized and casted on glassy carbon electrode (GCE). The synthesized PDA-co-PPY was characterized by Fourier transform infrared spectroscopy (FTIR), field emission scanning electron microscopy (FESEM), electrochemical impedance spectroscopy (EIS), energy dispersive x-ray spectroscopy (XEDS) and x-ray photoelectron spectroscopy (XPS). After the electrochemical synthesis and deposition on glassy carbon electrode, the mass of copolymeric film (PDA-co-PPY) deposited was 0.3 μg. As synthesized PDA-co-PPY modified GCE showed good response towards electrocatalytic oxidation of uric acid with low limit of detection (0.1μM, S/N =3), good linearity (0.5-40μM) and high sensitivity (2.1μAμMcm-2). It was also found that PDA-co-PPY modified GCE displayed stable current response for uric acid unaffected by common interferents (electroactive substances). Also, when used for uric acid detection in human serum and urine, it showed a good recovery of 90-110%. The developed PDA-co- PPY nanocomposite is proposed as a promising biosensor for uric acid for human health protection.